Bartosz Kędzierski, Wirginia Kukuła-Koch, Kazimierz Głowniak

Application of CPC and related methods for the isolation of natural substances – a review.
2014-03-27

A review of research on the isolation of various alkaloids from plant material by centrifugal partition chromatography (CPC) and related preparative techniques was made, in order to provide various conditions for separation of these important plant derived secondary metabolites. First of all, the construction of the CPC apparatus was presented as well as the principle of isolation of natural products with its help, and then the influence of operating apparatus parameters on the separation efficiency. Finally, a review of the alkaloids separation conditions was made, specifying used parameters and best solvent system.
Keywords: alkaloid separation, CPC separation, centrifugal partition chromatography, counter current chromatography