Israr Ahmad, Noshad Amir, Durr-E-Sabih, Muhammad Hassham Hassan Bin Asad, Muhammad Kashif Rahim, Muhammad Shahzad Hussain, Ghulam Murtaza, Syed Nisar Hussaian Shah

Preparation and radiochemical control of 99mTc labeled blood pool agent for in vivo labelling of the red blond cells.
2014-03-27

Our aim was to prepare cheap blood pool imaging kits by simplified method to overcome the burden on purchase department of MINAR, Nishtar Hospital, Multan, Pakistan. Secondarily, prompt supply of kits should save the time of patient during transportation. A total of 24 subjects selected for this study were equally divided into two groups. Mixture of stannous chloride and sodium pyrophosphate solution at pH 7 was injected to these subjects. Various concentrations (ranging from 200 to 800 ěg) of stannous chloride dihydrate were injected to group one, followed by intravenous administration of technetium-99m (99mTc) pertechnetate at 30 min interval in 12 subjects. Labeling percentage of each sample was calculated afterwards followed by imaging under ă camera. Each parameter was tested on three different patients and average of these three was calculated. In second set of experiments done on group two the same procedure was repeated in another 12 subjects, while keeping the concentration of Sn PYP constant at 400 ěg. In this case, 99mTc was administered at different time intervals in different subjects ranging from 15 to 120 min (15, 30, 60 and 120 min) followed by calculation of labeling percentage and imaging under ă camera. In group one, average percentage values of binding of red blood cells with 99mTc were 23.24, 84.88, 83.78 and 60.33% for concentrations of 200, 400, 600 and 800 ěg, respectively. In group two, average percentage binging values of 22.26, 84.36, 55.54 and 28.67% were calculated at time intervals of 15, 30, 60 and 120 min, respectively. It is concluded from the results that the best blood pool imaging under ă camera was observed for the concentration of 400 ěg and the time interval of 30 min. The maximum percentage binding of red blood cells with 99mTc was calculated at concentration of 400 ěg after 30 min interval that also correlated with imaging results.
Keywords: radiochemical control, 99mTc labeled blood pool agent, in vivo labeling, percentage binding of red blood cells with 99mTc